site stats

Tacc3 oocyte

WebTransforming acidic coiled-coil-containing protein 3 is a protein that in humans is encoded by the TACC3 gene. [5] [6] The function of this gene has not yet been determined; however, it is speculated that it may be involved in cell growth and differentiation. WebJul 15, 2024 · We speculated that the function of TPX2 beyond microtubule assembly is conserved among species and may also be related to AURKA and TACC3. Here, porcine oocytes were selected to reveal the spatiotemporal and function of TPX2 in microtubule …

Cobalt chloride exposure disturbs spindle assembly and

WebDec 19, 2016 · TACC3 was localized to the spindle poles in the control groups. However, it’s disrupted in the KIF2A siRNA-injected groups. Red TACC3, green α-tubulin, blue DNA. Scale bar = 20 μm. Full size... WebMar 1, 2024 · Cobalt chloride disrupted spindle formation by affecting TACC3 and decreasing Ac-a-tubulin levels, altered oocyte actin distribution and polarity, and impaired the mitochondrial structure and functions. The results provide in vivo evidence for cobalt chloride negatively affecting oocyte development. 2. Materials and methods 2.1. Chemicals saga business health insurance https://cartergraphics.net

Tacc3 transforming, acidic coiled-coil containing protein 3

WebSep 19, 2005 · TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A–dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis. … WebTACC3; transforming acidic coiled-coil containing protein 3. Aliases: ERIC1, Tacc4, ERIC-1, maskin. Location: 4p16.3. Summary: This gene encodes a member of the transforming acidic colied-coil protein family. The encoded protein is a motor spindle protein that may … WebApr 26, 2024 · In summary, we conclude that AURKA is the only Aurora kinase in mouse oocytes that is essential for fertility and MI [ 30, 31] ( Fig 8 ). Its unique functions include, at least, initiating MTOC fragmentation through activation of PLK1 and spindle formation … saga building society base rate

Self-assembly and sorting of acentrosomal …

Category:TACC3 (transforming, acidic coiled-coil containing protein 3)

Tags:Tacc3 oocyte

Tacc3 oocyte

TACC3 expression and localization in the murine egg and ovary

WebJul 13, 2015 · TACC3 mRNA was detected in bovine oocytes during meiosis using qRT-PCR, and while it was also expressed in cleavage stage embryos, its expression was down-regulated at the morula and blastocyst stages. Immunofluorescence was used to … WebSep 26, 2024 · Tacc3 is a negative regulator of the Notch signaling pathway. in addition to spindle assembly, Tacc3 has critical roles in chromosome separation and cytokinesis, and is essential for the mitosis of sclerotome mesenchymal cells during axial formation in mammals. role in hematopoietic stem cell function and interface with p53-regulated …

Tacc3 oocyte

Did you know?

WebApr 3, 2024 · TACC3 is related to biological progress (BP), cellular component (CC), and molecular function (MF). ... Alzheimer disease, oocyte meiosis, phagosome, cell cycle, cellular senescence, DNA replication, and progesterone-mediated oocyte maturation. The 2 most negatively related categories included lysosome and human T cell leukemia virus 1 ... WebMay 1, 2024 · During mammalian oocyte development, two contrasting patterns of polyadenylation have been identified. ... SLBP (Abnova H00007884-M01, 200 ng/mL), tubulin (Sigma T8203, 10 μg/mL) and TACC3 ...

WebJul 13, 2015 · TACC3 mRNA was detected in bovine oocytes during meiosis using qRT-PCR, and while it was also expressed in cleavage stage embryos, its expression was down-regulated at the mor-ula and... WebSep 26, 2024 · Tacc3 is a negative regulator of the Notch signaling pathway. in addition to spindle assembly, Tacc3 has critical roles in chromosome separation and cytokinesis, and is essential for the mitosis of sclerotome mesenchymal cells during axial formation in …

WebThe expression levels of several microtubuleassociated proteins including TACC3, KIF14 and ASPM were also reduced in the aging oocytes. ... Proteomic analysis implicates that postovulatory... WebNov 1, 2002 · A protein spot cored from a silver-stained two dimensional (2D) gel of germinal vesicle stage immature mouse oocytes was identified as Transforming Acidic Coiled Coil containing protein (TACC3)...

WebJul 13, 2015 · TACC3 is expressed in oocytes and in early cleavage stage pre-implantation embryos. The expression of TACC3 mRNA in bovine oocytes and in vitro produced pre-implantation embryos was examined using qRT-PCR. TACC3 expression was detected at similar levels (p>0.05) throughout oocyte maturation and during the first cleavage stages …

WebNational Center for Biotechnology Information saga by brian k. vaughan and fiona staplesWebOct 14, 2024 · The primary antibodies were listed as follows: MTX2 (1:100 in dilution, 11610-1-AP, Proteintech), GDF9 (1:100 in dilution, ab93892, Abcam), TACC3 (1:50 in dilution, ab134154, Abcam), EIF5A (1:50 in dilution, 11309-1-AP, Proteintech), ASPM (1:150 in dilution, 26223-1-AP, Proteintech). they upWebJun 28, 2024 · Abstract. Mammalian oocytes segregate chromosomes with a microtubule spindle that lacks centrosomes, but the mechanisms by which acentrosomal spindles are organized and function are largely unclear. In this study, we identify a conserved … the yunna serverWebFeb 25, 2024 · Oocyte treatments with taxol, nocodazole, or cold did not affect CLTC expression levels but led to disorders of its distribution. Functional impairment of CLTC by specific morpholino injections in GV-stage oocytes led to disruptions in spindle assembly and chromosomal alignment, accompanied by impaired first polar body (PB1) emissions. the yunion detroit miWebOct 16, 2006 · Proteins from 500 germinal vesicle (GV) and metaphase II- (MII) arrested oocytes were extracted, resolved on 2D electrophoretic gels, and stained with silver. Analysis of the gels indicated that 12 proteins appeared to … saga car insurance 3 year fixedWebthat TACC3 is required for acentrosomal microtubule assembly (Fig. 1 C and D and Fig. S1 C and D). To understand why TACC3 is required for the acentrosomal microtubule aster assembly, we treated the mitotic HeLa cells with nocodazole or cold temperatures and observed that TACC3 stayed with the stable kinetochore-connectedfibers the yung wing schoolWebTransforming acidic coiled-coil (TACC) proteins are key players during mitosis via stabilization of the spindle. The roles of TACCs during meiosis are however less clear. We used bovine oocytes... saga butler service