Pstat3 kit
WebApr 13, 2024 · Cellular viability was obtained through Cell Counting Kit-8 (CCK-8, 40203ES80; Yesean) as described by the manufacturer. Tested cells were seeded in 96-well plates (5000 cells/well), transfected with LINC00654 silencers via RNAiMAX and treated with RSL3 (1219810-16-8; MCE), erastin (571203-78-6; MCE), Ferrostatin-1 (Fer-1, … WebThe HTRF IL23/IL23R Binding kit is designed to measure the interaction between IL23 and IL23R proteins. Without treatment, IL23 binds to IL23R, and the binding of each detection antibody to its respective tagged protein generates an HTRF signal. In the presence of a compound or an anti-human blocking antibody, the IL23/IL23R interaction is ...
Pstat3 kit
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Web目的：本实验旨在研究扶正抗癌方对荷H22移植性肝癌细胞小鼠中STAT3及HIF-1α表达的影响，以探讨其抑制肿瘤侵袭转移的作用机制。 方法：采用细胞接种的方法建立小鼠H22肝癌动物模型，并将小鼠随机分为五组，空白对照组，荷瘤对照组，替加氟组，扶正抗癌方组，扶正抗癌方+替加氟组。观察各组 ... WebNifuratel, a novel STAT3 inhibitor with potent activity against human gastric cancer cells Hailun Zheng,1,2,* Huang Hong,1,* Lulu Zhang,1,2,* Xiong Cai,1,2 Meng Hu,1,2 Yuepiao Cai,2 Bin Zhou,1 Jiayuh Lin,3 Chengguang Zhao,2 Wanle Hu1 1Department of Coloproctology, The Second Affiliated Hospital and Yuying Children’s Hospital of …
WebChromatin immunoprecipitations were performed with cross-linked chromatin from Hep G2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes, and either … WebJul 2, 2024 · The scale bars, 50 µm. (D) primary human AML cells (n = 7) and controls (n = 2) were cocultured with bone marrow–derived primary human mesenchymal stem and progenitor cells. MFI of pSTAT3 and pSTAT5 of stromal cells assessed by BD Phosflow technology (each sample was tested in duplicate).
WebAffiliations 1 Medical Faculty, Institute of Clinical Immunology, University of Leipzig, Leipzig, Germany. Electronic address: [email protected]. 2 Medical Faculty, … WebTHUNDER™ Phospho-STAT3 (Y705) + Total STAT3 TR-FRET Cell Signaling Assay Kit. Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT3 …
WebFeb 18, 2024 · RNA was extracted using the RNeasy kit (QAIGEN, USA). Extracted RNA was converted to cDNA using GeneAmp (Applied Biosystems, USA). ... Following 24 h, there was also an increase in pSTAT3-S727 and SOCS3 levels (Fig. 1e-f). Table 1 List of primers. Full size table. Fig. 1.
WebMar 23, 2024 · The expression of pSTAT3 and the activation of the STAT3 signaling pathway (JAK2, STAT3, and pSTAT3) in the periodontal tissue and cortex were ... flight a3608WebApr 11, 2024 · The percentages of Ki-67-, caspase-3-, MYC- and pSTAT3-positive cells derived from histology scores were quantified in the three tumor sections of each treatment group and are shown in a bar diagram. ( D ) Luciferase-expressing HD-MB03 cells were implanted orthotopically into the cerebella of NSG mice for tumor growth and the mice … chemical equations and stoichiometryWebPSTAT3-TA-luc can be transfected into cells by conventional cell transfection method. The biyuntian firefly firefly firefly firefly luciferase reporter gene assay kit detection … flight a359WebDec 15, 2016 · p-Stat3 Antibody (B-7) is a mouse monoclonal IgG 2b κ, cited in 449 publications, provided at 200 µg/ml. Anti-p-Stat3 Antibody (B-7) is recommended for … chemical equation photosynthesis equationWeb10253-2-AP targets STAT3 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. signal transducer and activator of … flight a3855WebSep 13, 2024 · The pSTAT3 and total STAT3 protein levels in these cells were detected with western blot. (E) PD-L1 mRNA in these cells was detected by RT-qPCR. Results were … flight a3700Webp-STAT3 (Tyr705) Assay Kit . Products: ALSU-PST3-A500, ALSU-PST3-A10K, ALSU-PST3-A50K, ALSU-PST3-A-HV Kit-Specificity Information . This assay kit contains … flight a3654