Webwidespread use of the cyanine scaffold, the chemistry underpinning this “photoblueing” artifact had remained opaque. We recently found that the irradiation of the simplest pentamethine cyanine, 2, leads to formation of the corresponding trimethine cyanine, 3 (Fig. 1b).13 This reaction, involving the formal excision of ethene diradical from ... The dyes Alexa Fluor 647 (LifeTech, A20106), Cy5 (GE-Healthcare, PA15101), ATTO647N (ATTO-TEC, AD647N-31), CF660C (GE-Healthcare, SCJ4600050), ATTO565 (ATTO-TEC, AD565-31), and a custom-modified goat anti-rabbit IgG secondary antibody (Invitrogen, 31212) labeled with Alexa Fluor … See more Steady-state absorption and fluorescence emission spectra were recorded on a V-650 spectrophotometer (Jasco) and an FP-6500 spectrofluorometer (Jasco). Samples were … See more The photobleaching efficiency of fluorophores was analyzed at the ensemble level in a 0.3 mm path-length fluorescence cuvette (Hellma, 105.251-QS) using 10 µM dye solutions in PBS (Sigma, D8537-500ML), with … See more Fluorescence lifetime measurements were performed on a MicroTime200 (PicoQuant) time-resolved confocal fluorescence microscope setup consisting of a FLIMbee galvo … See more For analysis of the fluorescence lifetime of photoconverted Cy5 (GE-Healthcare, PA15101), the photobleached solution was purified using HPLC on a Kinetex biphenyl column (150 × 4.6 mm), consisting of 2.6 μm … See more
Bernhard Pelzl Inventions, Patents and Patent Applications
WebJul 1, 2012 · This study reported, for the first time systematically, photodegradation of Rhodamine B (RhB) in aqueous solution over BiOCl and BiOBr semiconductors. Under visible light irradiation (λ>400 nm, λ>420 nm and λ=550±15 nm), RhB adsorbed on the surface of BiOCl and BiOBr was photosensitized and decomposed effectively over unexcited BiOCl … WebMar 1, 2024 · Mar 01, 2024: How photoblueing disturbs microscopy (Nanowerk News) The latest developments in fluorescence microscopy make it possible to image individual … bishan towers progress
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WebJun 12, 2024 · An undesirable effect can occur in super-resolution fluorescence microscopy: photoblueing. A new publication in „Nature Methods“ shows how it can be prevented or made useful for research. WebOct 1, 2006 · Photoblueing of organic dyes can cause artifacts in super-resolution microscopy. 25 February 2024. Dominic A. Helmerich, Gerti Beliu, … Markus Sauer. WebFeb 26, 2024 · How photoblueing disturbs microscopy. Confocal fluorescence images of glass surfaces coated with the cyanine dyes Alexa Fluor 647 (a) and CF660C (b) and with … bishan wellness hub