Pearleygate 202
WebFeb 11, 2015 · PCR reactions were recombined into pEarleyGate 202 by LR recombination (Invitrogen) and populations of bacterial clones were pooled into libraries of random mutants. Prepared pEG202 ATR1-Cala2 from these libraries was transformed into Agrobacterium tumefaciens strain GV3101 by electroporation, and individual clones were … WebJul 12, 2024 · The pEarleyGate103 and the recombinant vector pEarleyGate103-SmSPL2 were transformed into onion epidermal cells, respectively, and the GFP signal was recorded, as we previously described [25]. ......
Pearleygate 202
Did you know?
WebJan 1, 2010 · The coding sequence of PKL, PKL-N, and PKL-C were amplified and cloned into the BamHI sites of the modified pEarleyGate 201-nYFP and pEarleyGate 202-cYFP vector by ClonExpress Entry One Step ... WebMar 1, 2016 · To construct vectors for co-IP analysis, CaCDPK15 or CaWRKY40 in the pDONR vector was directly introduced into the destination vectors p35S:HA (pEarleyGate 201) and p35S:Flag (pEarleyGate 202) to ...
WebpEarleyGate 202. Chloramphenicol 11776 pEarleyGate 203. Chloramphenicol 11783 ... pEarleyGate 304. Chloramphenicol 10379 pEcCas. Kanamycin 14605 pEcgRNA. Spectinomycin 3134 ... WebNov 22, 2024 · The ENTRY vector pDONR207- SmbHLH37 was sequenced and inserted into the pEarleyGate 202 vector ( Earley et al., 2006) by an LR reaction (Gateway ®) to generate the pEarleyGate 202- SmbHLH37 overexpression vector. Agrobacterium -mediated gene transfer was performed based on protocols established in our laboratory ( Yan and Wang, …
WebSuite 202 Charlotte, NC 28211. Hours. Monday to Friday 8 a.m. to 5 p.m. Contact Us. Phone 704-316-3070 Fax 704-316-3071. Appointments Our Providers Our Services Location. … Webwas sequenced and inserted into the pEarleyGate 202 . 138 . vector (Earley . et al., 2006) by an LR reaction (Gateway®) to generate the . 139 . pEarleyGate 202‒ SmbHLH37. overexpression vector. Agrobacterium-mediated gene . 140 . transfer was performed based on protocols established in our laboratory (Yan and . 141 . Wang, 2007). 142
WebFor BiFC analysis, the vectors pEarleyGate 201-YN and pEar-leyGate 202-YC were kindly supplied by Dr Yuhai Cui (Lu etal., 2010). We cloned the full-length open reading frame …
WebOct 7, 2024 · Plant diseases cause devastating crop losses around the world and threaten the food supply of millions of people. Over time, plants have developed various mechanisms for fighting off infections caused by pests and other pathogens such as viruses and bacteria. When plants become infected they kick their immune system into action by … hot tools dual plate flat ironWebpEarleyGate202载体质粒图谱、序列、价格、抗性、测序引物、大小等基本信息_生物风载体 pEarleyGate202 价格： 6500元 联系方式： I47-825O-882O 相关技术服务： 质粒构建 基因 … hot tools extended barrelWebpEarleyGate 202 allows for rapid recombinational cloning of cDNAs from Gateway(TM) entry vectors to be epitope tagged with FLAG tag at the N-terminus of the encoded protein. This … hot tools electric curlersWebNewborn and well-child maintenance. In-office X-rays and labs. Minor procedures. Immunizations. School physicals. Sports medicine. Youth sport physicals. 9908 Couloak … line rider marriage of figaroWebJan 26, 2006 · Gateway cloning technology facilitates high-throughput cloning of target sequences by making use of the bacteriophage lambda site-specific recombination … line rider beethoven\\u0027s 5thWebJan 1, 2004 · We amplified the sequence containing MCS from the pUC-SPYNE plasmid (Walter et al., 2004) and used it to replace the ccdB sequence between attR1 and attR2 sites in the pEarleyGate 201-nYFP and... hot tools extended barrel curling ironWebApr 30, 2004 · 202 Parkgate Dr was built in 1994 and last sold on April 30, 2004 for $277,000. What is the rental estimate for this home? We estimate that 202 Parkgate Dr … hot tools electric rollers