Hidi polymerase
WebHiDi® stands for High Discrimination of nucleotides mismatching at the 3’-terminus of PCR primers. The HiDi® 2X PCR Master Mix is a SYBR Green-compatible ready to use mix that contains a highly selective recombinant DNA polymerase suitable for allele-specific PCR (AS-PCR)1, primer extension or methylation-specific PCR. WebHiDi® Taq 2x PCR Master Mix Datasheet Catalog Number: #4200 Powered by Bioz Skip to the end of the images gallery Skip to the beginning of the images gallery Key Features …
Hidi polymerase
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WebThe 5′ to 3′ exonuclease activity of the repair enzyme DNA polymerase I may remove additional nucleotides, whereas its polymerase activity allows the insertion of the normal … WebHiDi® Taq DNA Polymerase 1000 U INCLUDING SEPARATE REACTION BUFFER $ 315.00 Using HiDi, less than 10 mutations can be detected in a background of >10.000 …
WebThe proposed method is a modified and improved version of the existing “Allele-specific q-PCR” (ASQ) method for genotyping of single nucleotide polymorphism (SNP) based … WebHiDi Taq DNA polymerase is a highly selective DNA polymerase variant, specially evolved for all assays in which High Discrimination is required, for instance in allele-specific PCRs, primer extensions or methylation-specific PCRs.
WebHiDi® Taq DNA polymerase #9201, Manual Version 6, May 20, 2024 Store at -20°C. Contents HiDi® (High Discrimination) Taq DNA polymerase, 5 U/µl HiDi® reaction buffer, 10x Description HiDi® Taq DNA polymerase is a highly selective DNA polymerase variant, specially evolved for all assays in which Web• HiDi DNA polymerase can also be used for realtime cycling when adding a suitable realtime PCR fluorescence dye). • HiDi DNA polymerase is a nuclease deficient DNA …
Using HiDi DNA polymerase, less than 10 mutations can be detected in a background of >10.000 wild-type sequences without any other modifications. HiDiTaq works also with hydrolysis probes. HiDi is the ideal enzyme for mutation detection, SNP genotyping, HLA typing. Also freeze-dried and/or customized available.
WebHiDi® stands for High Discrimination of nucleotides mismatching at the 3’-terminus of PCR primers. The HiDi® 2X PCR Master Mix is a SYBR Green-compatible ready to use mix … golf swing uneven groundWebThe primer design for the HvSAP16 gene of barley, with an amplicon size of 83-bp, is presented in Figure 2. ASPs consist of a set of three oligonucleotides: two forward primers and one common reverse primer, each around 22 bp in length, as usual for PCR primers ( … health care business ideasWebHiDi® is the best choice for highly selective PCRs, such as allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a … healthcare business insights hbiWeb1 gen 2024 · Therefore, HiDi DNA polymerase was used to improve the amplification efficiency of the DNA fragments using PCR (step 3). HiDi DNA polymerase is a highly selective DNA polymerase specifically evolved for assays that require high single nucleotide identity and efficient identification of primers with a mismatch at the 3′ end ... healthcare business insights loginWebHiDi and HiDi Taq DNA polymerase is hotstart-formulated with an aptamer. Temperatures above 50-55°C cause the aptamer’s secondary structure to melt and will set-free the polymerase. HiDi is supplied as a 5 U/µl solution containing glycerol and is supplied together with 10x reaction buffer which has been optimized for short amplicons between … golf swing vest trainerWebHiDi® DNA polymerase is a highly selective DNA polymerase variant, specially evolved for all assays in which High Discrimination is required, for instance in allele-specific PCRs, … golf swing uppercutWeb• HiDi 10X buffer is optimized for short amplicon length (about 60 – 200bp), but also longer amplicons are possible. The addition of additional MgCl2 (0.5 – 1.5mM) might be needed in case of longer amplicons (>500bp). • HiDi DNA polymerase can also be used for realtime cycling when adding a suitable realtime PCR fluorescence dye). golf swing using hands and wrists