Flag tag mouse monoclonal antibody

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August undefined, 2024

WebThe antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies) fused to either the amino- or carboxy-terminus of targeted proteins. The binding specificity of this … WebAnti-DYKDDDDK (FLAG® epitope Tag) Antibody, Mouse Monoclonal Images Immunofluorescence staining of flag-Tag in 293 cells, transfected with PCMV-CDH1-flag (Figure A), pSTEP2-Flag-FABP4-GST (Figure B), pSTEP2-Flag-ARG1-GST (Figure C), Flag-PRMT5-His (Figure D),Flag-PRMT6-His (Figure E) and His-flag-CD38 (Figure F).

Flag Tag Monoclonal Antibody - Cusabio

WebAnti-FLAG Antibody Products The following lists a number of FLAG antibodies from various suppliers. The target antigen refers to the FLAG epitope tag, which is an octapeptide containing the amino acid sequence DYKDDDDK. Such antibodies can be used to detect or stain Flag-tagged recombinant proteins. Examples of FLAG antibody … WebDYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology. Protocols Publications All (44) WB (29) IF (2) IP (11) CoIP (8) phillip burkhart

Anti-MMP-9 Antibody, Mouse Monoclonal - Sino Biological

WebSpecificity: Flag-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal Flag-tagged proteins. Immunogen: A synthesized peptide. Western blot analysis of Flag-tagged protein expression in (1) 293T cell lysate; (2) 293T cell transfected with Flag-tagged protein lysate. β-Actin Rabbit Polyclonal Antibody WebFlag Tag Monoclonal Antibody Datasheet Product Details Citations Customer Reviews and Q&A Product Details Citations Stomatin modulates adipogenesis through the ERK pathway and regulates fatty acid uptake and lipid droplet growth SC Wu,Nature communications,2024 WebKey features and details PE Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt, Sandwich ELISA Reacts with: Species independent Conjugation: PE. Ex: 488nm, Em: 575nm Isotype: IgG1 Get better batch-to-batch reproducibility with a recombinant antibody phillip burkhalter builders rome ga

DYKDDDDK Tag Monoclonal Antibody (FG4R), DyLight™ 488

HA Tag Mouse mAb(HA Tag 小鼠单抗)(AF2858-50μl)

WebOct 4, 2024 · The FLAG-tagged protein identifies and binds to the FLAG tag-specific monoclonal antibody conjugated on a magnetic bead or agarose gel. Then, residual impurities are washed away, and the FLAG-tagged proteins are eluted using an affinity column with a low pH buffer or a high concentration of FLAG tag peptide. WebOur DYKDDDDK Tag monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit, Rat, Goat and Chicken. These … try my smart petWebAnti-DYKDDDDK (FLAG® epitope Tag) Antibody Features Suitable for Western Blot / WB application Broad-spectrum Recognition Capability Detect N and C terminal DYKDDDDK Tag in fusion proteins Anti-DYKDDDDK mouse monoclonal antibody at 1:1000 dilution. Sample: Recombinant Protein, 10ng/lane Lane A to K: Proteins with C-terminal FLAG® Tag try my south east mix it\u0027s a poser

"WebJan 21, 2024 · For the primary antibody reaction, DYKDDDDK Tag (D6W5B) Rabbit monoclonal antibody (1:1000, 14793; Cell Signal Technology, Beverly, MA, USA) and anti-C23 (NCL) mouse monoclonal (1:500, sc-55486; Santa Cruz Biotechnology, Dallas, TX, USA) were used in the antibody solution, and the mixture was allowed to react with the … " - Flag tag mouse monoclonal antibody

Flag tag mouse monoclonal antibody

DYKDDDDK Tag Antibody (Binds to same epitope as …

WebMonoclonal ANTI-FLAG® M2 antibody produced in mouse clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium … Web4C5 Anti-DDK Antibody - OriGene’s anti-tag antibodies are developed with high standards of production procedure and validation. For example, 4C5 anti-DDK antibody is generated to detect the DYKDDDDK epitope (same epitope as Flag®). This antibody has been verified for Western blot, immunoprecipitation, immunofluorescence and flow cytometry.

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WebMany recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This antibody can detect either N-term or C-term HA tag. 产品编号. 产品名称. 包装. AG8057. HA Tag Rabbit Monoclonal Antibody. 50µl. AZ050. http://www.abpbiosciences.com/product/flag-tag/

WebThe DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this … Web保存条件： HA Tag Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。

WebDescription: Antibody for the detection of FLAG™ conjugated proteins (MOUSE) Monoclonal Antibody - 200-301-B13 Synonyms: mouse anti-FLAG™ tag, Enterokinase Cleavage Site (ECS), mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys Tested Applications: ELISA, WB Suggested Applications: FC, IF, IP - View References … WebNeuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and the production of antibodies by B cells. We established monoclonal mouse anti-mouse neuritin antibodies by immunizing knock-out mice with two different neuritin-derived …

WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence.

WebFlag Tag monoclonal antibody CSB-MA000021M0m was produced in the mouse immunized by using the DYKDDDDK(Flag) synthetic peptide conjugate to KLH as the … try my side of love chi litesWebanti-Flag-Tag mouse mAb Conjugated Agarose beads is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Size: 1 ml Concentration: 50% Slurry Availability: In stock 505.46 594.66 USD505.46 Size: 500 µl phillip burkholderWebDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) DDDDK tag Monoclonal Antibody for ELISA. Host / Isotype. Mouse / IgG2b. Reactivity. human, mouse, rat . ... try my speedWebThis antibody can also be used in the immunohistochemical analysis of mouse tissues containing FLAG peptide without any background staining (PMID: 28377223). 2H8 was … phillip burksWebMouse monoclonal [9E10] to Myc tag Suitable for: ICC/IF, Flow Cyt, WB, IP, ELISA, IHC-Fr, Purification Reacts with: Recombinant fragment Isotype: IgG1 Related conjugates and formulations Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 APC Biotin Biotin DyLight® 488 DyLight® 550 DyLight® 650 FITC HRP PE SureLight® APC See all (13) try my solutionhttp://www.engibody.com/products/DYKDDDDK-FLAG-tag-Mouse-mAb-conjugated-Agarose-Beads-AT0078.html phillip burlingWebNeuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and … try mysql